Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10216/103063
Autor(es): | Rita S. Santos George R. Dakwar Ranhua Xiong Katrien Forier Katrien Remaut Stephan Stremersch Nuno Guimarães Sílvia Fontenete Jesper Wengel Marina Leite Céu Figueiredo Stefaan C. De Smedt Kevin Braeckmans Nuno F. Azevedo |
Título: | Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori |
Data de publicação: | 2015 |
Resumo: | Helicobacter pylori infects more than 50% of the worldwide population. It is mostly found deep in the gastric mucus lining of the stomach, being a major cause of peptic ulcers and gastric adenocarcinoma. To face the increasing resistance of H. pylori to antibiotics, antimicrobial nucleic acid mimics are a promising alternative. In particular, locked nucleic acids (LNA)/2'-OMethyl RNA (2'OMe) have shown to specifically target H. pylori, as evidenced by in situ hybridization. The success of in vivo hybridization depends on the ability of these nucleic acids to penetrate the major physical barriers-the highly viscoelastic gastric mucus and the bacterial cell envelope. We found that LNA/2'OMe is capable of diffusing rapidly through native, undiluted, gastric mucus isolated from porcine stomachs, without degradation. Moreover, although LNA/2'OMe hybridization was still successful without permeabilization and fixation of the bacteria, which is normally part of in vitro studies, the ability of LNA/2'OMe to efficiently hybridize with H. pylori was hampered by the presence of mucus. Future research should focus on developing nanocarriers that shield LNA/2'OMe from components in the gastric mucus, while remaining capable of diffusing through the mucus and delivering these nucleic acid mimics directly into the bacteria. |
URI: | https://hdl.handle.net/10216/103063 |
Informação Relacionada: | info:eu-repo/grantAgreement/Autoridade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2 info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projectos de Investigação Clínica/PIC/IC/82815/2007/Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos/DNA mimics info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE |
Tipo de Documento: | Artigo em Revista Científica Internacional |
Condições de Acesso: | openAccess |
Aparece nas coleções: | FEUP - Artigo em Revista Científica Internacional FMUP - Artigo em Revista Científica Internacional |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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143176.pdf | Artigo original publicado | 1.62 MB | Adobe PDF | Ver/Abrir |
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