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Effects of green light and Polypodium leucotomos extract on melanoma suppression

Wed, 01 Jan 2025 00:00:00 GMT

Title: Effects of green light and Polypodium leucotomos extract on melanoma suppression

Ultrafiltration rate and interleukin-6 levels are independent predictors of erythropoietin resistance in chronic hemodialysis patients

Wed, 01 Jan 2025 00:00:00 GMT

Title: Ultrafiltration rate and interleukin-6 levels are independent predictors of erythropoietin resistance in chronic hemodialysis patients Abstract: Abstract Background and Aims Anemia is common in patients under regular hemodialysis therapy. Despite treatment with erythropoiesis-stimulating agents (ESA), hypo-responsiveness to this therapy can occur (refractory cases). We studied the association between ESA resistance in hemodialysis patients and potential causes of ineffective treatment, including nutritional, iron metabolism, and inflammatory variables, and dialysis related parameters. Method We performed a cross-sectional study involving 289 hemodialysis patients. Ultrafiltration rate adjusted to weight (UFR/W), urea reduction ratio (URR) and Kt/V were obtained for the session where blood samples were collected. Blood analyses were performed by using automated technology or by enzyme-linked commercialized immunoassays, as previously described [1]. Erythropoietin (EPO) resistance index (ERI) was calculated by the formula: EPOdoseperweek (IU)/bodyweight (kg)/hemoglobin (g/dL). Results The mean age of patients was 68.7 years (±13.6), 240 patients (83%) were under ESA prescription and 186 (64.4%) were receiving intravenous iron. The median (interquartile range) for ERI was 7.1 (3.711.7) IU/kg/g/dL, and for median transferrin saturation (TSAT) was 21.9 (16.828.5) %. ERI was positively correlated with levels of soluble transferrin receptor (sTfR, r = 0.556, P = 7.8×1021; Fig. 1), interleukin (IL)-6 (r = 0.196, P = 0.002), pentraxin 3 (r = 0.192, P = 0.003), C-reactive protein (r = 0.130, P = 0.043) and UFR/W values (r = 0.135, P = 0.037) and inversely correlated with body mass index (r = 0.179, P = 0.005) and levels of iron (r = 0.382, P = 9.2×1010), TSAT (r = 0.340, P = 6.8×108), hepcidin (r = 0.197, P = 0.002) and albumin (r = 0.166, P = 0.010). By performing multivariate linear regression analysis (after normalization of non-normally distributed data), levels of sTfR (ß = 0.487, P < 0.001), iron (ß = 0.176, P = 0.003), and IL-6 (ß = 0.111, P = 0.035), and UFR/W values (ß = 0.168, P = 0.001) were independent predictors of ERI. When iron repleted patients (ferritin 100 µg/L and TSAT 20%) receiving EPO therapy were analyzed separately (n = 138), sTfR and UFR/W were kept in the regression model as predictors of ERI (ß = 0.504, P < 0.001 and ß = 0.207, P = 0.006, respectively); within these patients, those with UFR/W > 10 ml/h/kg presented higher ERI than those below 10 ml/h/kg (Fig. 2). Conclusion Besides lower iron, raised UFR/W and IL-6 are independently associated with hypo-responsiveness to ESA. sTFR seems to be a valuable tool to access ESA-driven erythropoiesis in hemodialysis patients, particularly in iron repleted patients. Our results support the idea that the modulation of inflammation and UFR prescription can improve anemia management.

CIRCULATING BETA-TRACE PROTEIN VERSUS CREATININE AS MARKERS OF EARLY KIDNEY (DYS)FUNCTION IN THE NX-INDUCED RAT MODEL OF CHRONIC KIDNEY DISEASE

Sun, 01 Jan 2023 00:00:00 GMT

Title: CIRCULATING BETA-TRACE PROTEIN VERSUS CREATININE AS MARKERS OF EARLY KIDNEY (DYS)FUNCTION IN THE NX-INDUCED RAT MODEL OF CHRONIC KIDNEY DISEASE Abstract: Abstract Background and Aims Beta trace protein (BTP) is a low molecular weight protein that has been proposed as an earlier biomarker of decreased glomerular filtration rate (GFR) and renal damage, than the traditional biomarkers, particularly in the creatinine blind range. Early biomarkers of renal (dys)function are needed to allow, in due time, the detection and treatment, to prevent worsening of the disease. To the authors knowledge, neither urine nor serum BTP has been assessed in animals with kidney disease. In the present study, we aimed to concomitantly evaluate BTP and creatinine circulating levels, to compare their value as early biomarkers of renal dysfunction, by performing the studies in rat models of mild and moderate chronic renal failure (CRF) induced by nephrectomy. Method Male Wistar rats, 12 weeks old, were randomly divided in three groups: Sham (n = 8, subjected to surgical process without kidney mass reduction), Mild CRF (n = 8, subjected to 1/2 nephrectomy), and Moderate CRF (n = 7, subjected to 5/6 nephrectomy). After five weeks, rats were sacrificed, blood and kidneys were collected. We analysed the circulating levels of BTP and creatinine and studied the association of BTP concentration with the glomerular and tubulointerstitial lesions, and with the traditional biomarkers of renal (dys)function, eGFR and creatinine. Results The serum levels of BTP were correlated with serum levels of creatinine (r = 0.575, p = 0.004) and also with eGFR (r = -0.453, p = 0.030); additionally, we found positive correlations with the total score of mild and advanced tubular lesions (r = 0.610, p = 0.02 and r = 0.517, p = 0.011, respectively), observed in kidney sections. The circulating levels of BTP increased with disease severity; Mild CRF showed a higher value of BTP than Sham group, although without statistical significance that was reached in Moderate CRF group, as observed for serum creatinine. The combined use of BTP and creatinine did not improve the discriminatory power in early disease detection. Though, the combination showed stronger correlations with the total score of tubular lesions (r = 0.849, p < 0.001 for mild tubular lesions and r = 0.774, p < 0.01 for advanced tubular lesions). Conclusion In rat models of mild and moderate CRF induced by nephrectomy, serum BTP levels increased with kidney function worsening, and correlated with disease severity, assessed by GFR and the degree of histopathological alterations. However, the earlier diagnostic value of BTP does not outperform serum creatinine in this model.

Unveiling the therapeutic potential of cannabinoids in ER+ breast cancer: cytotoxicity and endocrine activity profile

Mon, 30 Sep 2024 00:00:00 GMT

Title: Unveiling the therapeutic potential of cannabinoids in ER+ breast cancer: cytotoxicity and endocrine activity profile

A comparative study of the cytotoxic effects of cannabidiol and minor cannabinoids on placental trophoblast cells

Sun, 08 Sep 2024 00:00:00 GMT

Title: A comparative study of the cytotoxic effects of cannabidiol and minor cannabinoids on placental trophoblast cells

TUMOR NECROSIS FACTOR RECEPTOR 2 POLYMORPHISM (RS1061622) AND INFLAMMATORY RESPONSE IN END-STAGE RENAL DISEASE PATIENTS UNDER DIALYSIS

Sun, 01 Jan 2023 00:00:00 GMT

Title: TUMOR NECROSIS FACTOR RECEPTOR 2 POLYMORPHISM (RS1061622) AND INFLAMMATORY RESPONSE IN END-STAGE RENAL DISEASE PATIENTS UNDER DIALYSIS Abstract: Abstract Background and Aims Enhanced levels of soluble tumor necrosis factor receptor 2 (sTNFR2) are known to associate with progressive chronic kidney disease (CKD), and is pointed as a potential biomarker for early detection of CKD; moreover, it has been reported as an independent predictor of all-cause mortality in end-stage renal disease (ESRD) patients under dialysis. Despite the increase in TNFR2 and in other inflammatory markers, recognized as risk factors for mortality in dialysis patients, the hypothesis that genetic polymorphisms of those biomarkers might modulate the inflammatory response and, thereby, the patients survival predisposition, has been poorly studied. Concerning TNFR2 genetic variants, a single nucleotide polymorphism in TNFR2 (+ 676 T/G; rs1061622), that results in amino acid change at position 196 (Met/Arg), was associated with higher levels of sTNFR2 in inflammatory conditions. The aim of this study was to determine the allelic frequencies of TNFR2 in ESRD patients and controls, and to evaluate its relationship with the circulating levels of inflammatory biomarkers. Method We studied 277 ESRD patients on dialysis and 32 controls, matched for gender, body mass index, and, as far as possible, for age. Real time PCR TaqMan SNP genotyping assay was used to assess allelic frequencies of TNFR2 (rs1061622). We also evaluated the circulating levels of TNF-alpha, sTNFR2, ferritin, hepcidin, elastase and cell-free DNA (cfDNA). Deaths occurring along 1-year follow-up period were recorded and mortality rates were assessed. Results ESRD patients presented higher levels of all studied biomarkers, as compared to controls; their overall mortality rate was 10.5%. Allelic frequencies in ESRD patients and controls were similar for TNFR2 (rs1061622) considering the homozygous and heterozygous individuals (2, p = 0.518). Concerning sTNFR2 values, no significant differences were observed between patients with genotypes TT, GG or TG. The GG genotype patients, compared to TT genotype carriers, presented significantly lower ferritin (p = 0.048), hepcidin (p = 0.038), elastase (p = 0.006) and cfDNA levels (p = 0.016); and compared to TG genotype patients, showed significantly lower ferritin (p = 0.039) and a trend towards lower values of hepcidin (p = 0.097), elastase (p = 0.079) and cfDNA (p = 0.0164). TG genotype patients showed higher TNF-alpha (p = 0.049) and a trend towards lower elastase (p = 0.081) than TT genotype subjects. The GG genotype patients presented a trend towards lower mortality rate (6.3%, 7.5%, and 12.4% for GG, TG and TT, respectively). Conclusion No differences were found in the allelic frequencies between controls and ESRD patients. The GG genotype patients for TNFR2 rs1061622 polymorphism showed decreased levels of inflammation, suggesting a more favorable inflammatory response, which is usually associated to a lower mortality risk in these patients. In accordance with the scientific community, recommending studies on genetic survival predisposition in dialysis patients, the polymorphisms of TNFR2 and of other inflammatory biomarkers deserve further studies. Acknowledgements: This work was financed by CESPU, through the project SNPsCKD-GI2-CESPU-2022; FCT, through the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular BiosciencesUCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomyi4HB.

ASSOCIATION BETWEEN INDEXING METHODS AND MEDICAL SUBJECT HEADINGS (MESH) ATTRIBUTED TO RESEARCH ARTICLES

Mon, 01 Jan 2024 00:00:00 GMT

Title: ASSOCIATION BETWEEN INDEXING METHODS AND MEDICAL SUBJECT HEADINGS (MESH) ATTRIBUTED TO RESEARCH ARTICLES

PERFORMANCE OF THE MEDICATION ADHERENCE UNIVERSAL QUESTIONNAIRE (MAUQ) IN PATIENTS UNDER ORAL ANTINEOPLASTIC MEDICATION

Mon, 01 Jan 2024 00:00:00 GMT

Title: PERFORMANCE OF THE MEDICATION ADHERENCE UNIVERSAL QUESTIONNAIRE (MAUQ) IN PATIENTS UNDER ORAL ANTINEOPLASTIC MEDICATION

VALIDATION OF HIGH RISK CRITERIA IN MEDICATION RECONCILIATION IN MAJOR ORTHOPEDIC SURGERY: A DELPHI STUDY

Sun, 01 Jan 2023 00:00:00 GMT

Title: VALIDATION OF HIGH RISK CRITERIA IN MEDICATION RECONCILIATION IN MAJOR ORTHOPEDIC SURGERY: A DELPHI STUDY

The Effect Of Age On Contraction-induced Glucose Uptake, Glut1 And Glut4 Expression In Skeletal Muscle

Fri, 01 Jan 2010 00:00:00 GMT

Title: The Effect Of Age On Contraction-induced Glucose Uptake, Glut1 And Glut4 Expression In Skeletal Muscle

P2 receptor-subtypes in the modulation of reactive gliosis

Tue, 01 Jan 2008 00:00:00 GMT

Title: P2 receptor-subtypes in the modulation of reactive gliosis

SAR, Site-Directed Mutagenesis and cell proliferation studies on new steroid aromatase inhibitors

Tue, 01 Jan 2008 00:00:00 GMT

Title: SAR, Site-Directed Mutagenesis and cell proliferation studies on new steroid aromatase inhibitors

Glial P2Y receptors contribute to the regulation of noradrenergic transmission in the rat brain cortex

Sun, 01 Jan 2012 00:00:00 GMT

Title: Glial P2Y receptors contribute to the regulation of noradrenergic transmission in the rat brain cortex

Glucose Uptake And Fiber Damage In Skeletal Muscle Stimulated With Different Frequencies

Thu, 01 Jan 2009 00:00:00 GMT

Title: Glucose Uptake And Fiber Damage In Skeletal Muscle Stimulated With Different Frequencies

Inhibition of astroglia proliferation by nucleotides: contribution of P1 receptors

Fri, 01 Jan 2010 00:00:00 GMT

Title: Inhibition of astroglia proliferation by nucleotides: contribution of P1 receptors

Guia para o estabelecimento de critérios microbiológicos em géneros alimentícios

Sun, 01 Jan 2017 00:00:00 GMT

Title: Guia para o estabelecimento de critérios microbiológicos em géneros alimentícios Editors: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP Description: O objetivo do Guia é compilar, complementar e disponibilizar um conjunto informação existente relativa aos critérios microbiológicos ao longo de todas as etapas da cadeia alimentar, não incluindo a produção primária, visando apoiar e facilitar a sua aplicação. O presente guia apresenta alguns exemplos de critérios microbiológicos aplicáveis ao longo da cadeia alimentar, a nível internacional, não dispensando a consulta da legislação em vigor. O presente documento destina-se a apoiar, entre outros, os operadores da indústria alimentar, da restauração, de laboratórios de controlo da qualidade, a comunidade científica e as entidades oficiais que atuem neste âmbito de atividade, sem prejuízo da aplicação de outros requisitos normativos e regulamentares. Autoria/Colaboração: Ana Lúcia Baltazar - ESTeSC; Ana Santos - VETDIAGNOS, Lda.; Maria Cecília Alexandre - ERSAR; António Lopes João - CMMV-Exército Português; Marta Ferreira - ATIVE, Lda.; Carla Novais - FFUP; Maria Cândida Marramaque - ANIL; Carlos Brandão - ESHTE; Patrícia Antunes - FCNAUP; Catarina Leitão Proença - CFPSA; Paulo Fernandes - INSA, I.P.; Cátia Martins Vaz - Dan Cake Portugal, S.A.; Pedro Caiado de Sousa - DGAV; Elisa Carrilho - ASAE; Rita Amaral Ferreira - Eurest Portugal, Lda.; Fátima Cordeiro - DGAV; Rita Temtem - LRVSA_Madeira; Helena Barroso - ISCSEM; Roberto Brazão - INSA, I.P.; Liliana Carvalho - Câmara Municipal de Braga; Silvia Viegas - INSA, I.P.; Luís Amaro - Ordem dos Nutricionistas; Sónia Pedro - IPMA, I.P.; Luísa Oliveira - INSA, I.P.; Sandra Quinteira - ESSVA-CESPU; Manuela Sol - ASAE; Verónica Ribeiro - CMMV-Exército Português; Márcia Reto - ASAE. O conteúdo desta publicação é da responsabilidade do Grupo de Trabalho Ocorrência Microbiológica na Cadeia Alimentar (GTOMCA), do Programa PortFIR, não representando, em termos jurídicos, a posição oficial das entidades e empresas que o compõem. Assim, estas não assumem qualquer responsabilidade ou obrigação por eventuais erros ou imprecisões que possam existir e a responsabilidade pela interpretação e uso do documento é exclusivamente do leitor. Ligação para o registo no repositório INSA. Editor: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP

Evaluation of the biochemical activity of new modified steroids as aromatase inhibitors

Thu, 01 Jan 2004 00:00:00 GMT

Title: Evaluation of the biochemical activity of new modified steroids as aromatase inhibitors

Using different anticholinergic lists for DBI calculation: effects on anticholinergic outcome prediction

Wed, 01 Jan 2020 00:00:00 GMT

Title: Using different anticholinergic lists for DBI calculation: effects on anticholinergic outcome prediction

Haverá diferenças nutricionais entre produtos de pastelaria com e sem glúten? Are there differences from a nutritional point of view between bakery products with and without gluten?

Fri, 01 Jan 2016 00:00:00 GMT

Title: Haverá diferenças nutricionais entre produtos de pastelaria com e sem glúten? Are there differences from a nutritional point of view between bakery products with and without gluten?

Association between patient's knowledge about hypertension with beliefs about medicines and medication adherence

Wed, 01 Jan 2020 00:00:00 GMT

Title: Association between patient's knowledge about hypertension with beliefs about medicines and medication adherence