<?xml version="1.0" encoding="UTF-8"?>
<feed xmlns="http://www.w3.org/2005/Atom" xmlns:dc="http://purl.org/dc/elements/1.1/">
  <title>DSpace Collection:</title>
  <link rel="alternate" href="https://hdl.handle.net/10216/73073" />
  <subtitle />
  <id>https://hdl.handle.net/10216/73073</id>
  <updated>2026-08-06T12:22:24Z</updated>
  <dc:date>2026-08-06T12:22:24Z</dc:date>
  <entry>
    <title>Targeting quorum sensing: ferulic and sinapic acids compromise Pseudomonas aeruginosa biofilm architecture and virulence</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/169479" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/169479</id>
    <updated>2026-04-22T06:40:54Z</updated>
    <published>2025-05-27T00:00:00Z</published>
    <summary type="text">Title: Targeting quorum sensing: ferulic and sinapic acids compromise Pseudomonas aeruginosa biofilm architecture and virulence</summary>
    <dc:date>2025-05-27T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Polyfluoroalkyl Substances (PFASs) Detection Via Carbon Dots: A Review</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/158318" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/158318</id>
    <updated>2025-08-27T06:22:48Z</updated>
    <published>2023-01-01T00:00:00Z</published>
    <summary type="text">Title: Polyfluoroalkyl Substances (PFASs) Detection Via Carbon Dots: A Review
Abstract: PFASs are a class of highly persistent chemicals that are slowly infiltrating soils and waterways. Thus, there is a great need for fast, sensitive, and reliable techniques to detect PFASs. Conventional methods, such as LC-MS/SPE, allow high sensitivities. However, such methods can be complex and expensive. Considering this, it is not surprising that the scientific community has turned their attention to the search for alternatives. New types of PFAS sensors have been reported over the years, being generally part of three classes: optical, electrochemical, or hybrid sensors. Carbon dots (CDs) are new alternative fluorescent sensors that can present great affinity towards PFASs, while allowing for a fast response and promising sensitivity and selectivity. Furthermore, CDs have more attractive properties than traditional fluorophores and even metal-based nanomaterials that make them better candidates for sensing applications. Thus, CDs display great potential for permitting a fast and accurate quantification of PFASs. This review aims to serve as a basis for the future development and optimization of CD-based fluorescent sensors for PFASs.</summary>
    <dc:date>2023-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Evaluation of the carbon footprint of the life cycle of wine production: A review</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/143656" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/143656</id>
    <updated>2025-08-27T06:22:47Z</updated>
    <published>2022-07-21T00:00:00Z</published>
    <summary type="text">Title: Evaluation of the carbon footprint of the life cycle of wine production: A review
Abstract: The accounting and reporting of greenhouse gas emissions associated with wine production have been increasingly pursued by the wine sector, due to increasing interest regarding environmental issues and sustainable development by consumers and organizations. Herein, this report aims to review and evaluate the existent literature regarding the calculation of carbon footprints at various stages of the life cycle of wine production. With this information, this report aims to improve the environmental performance of this sector in terms of greenhouse gas emissions. We have found widely variable carbon footprints between reviewed studies, which included different wine types from major wine producing countries and regions. We were also able to identify the life cycle stages that contribute the most to the overall carbon footprint of wine production, which are bottling and viticulture, while understanding which are the major hotspots at each stage. This allowed us to provide suggestions and recommendations for mitigation of greenhouse gas emissions. Thus, this report can guide future studies aiming to account, report and mitigate the carbon footprint of wine production.</summary>
    <dc:date>2022-07-21T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Optimizing Exploration: Synergistic approaches to minimize false positives in pegmatite prospecting - A comprehensive guide for remote sensing and mineral exploration</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/165747" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/165747</id>
    <updated>2025-03-14T07:21:14Z</updated>
    <published>2024-01-01T00:00:00Z</published>
    <summary type="text">Title: Optimizing Exploration: Synergistic approaches to minimize false positives in pegmatite prospecting - A comprehensive guide for remote sensing and mineral exploration
Abstract: Pegmatites represent a critical source of raw materials for the European Union's future, emphasizing the need to explore new deposits. Remote sensing plays a pivotal role in early-stage pegmatite exploration, generating prospectivity maps to facilitate field validation. The higher the false positive rate, the higher the time and costs required for field campaigns. This study aims to devise an approach that minimizes false positives, optimizing the work of the exploration field team. The proposed method seeks to create mineral prospectivity maps that accurately pinpoint high potential areas for pegmatite occurrences with minimal false positives occurrence through the application of fuzzy logic and leveraging the best mapping methods identified in prior research, in the Tysfjord region, northern Norway. Data from two multispectral satellites, namely WorldView-3 (WV3) and Sentinel-2, as well as light detection and ranging (LiDAR) data point cloud, were used, the last allowing the creation of a high-resolution hillshade map. Two classification methods were applied to the satellite images to leverage their individual advantages while minimizing possible weaknesses: the Mixture Tunned Matched Filtering (MTMF) using the Spectral Hourglass Wizard (SHW) Workflow and boosting through the LightGBM (LGBM) algorithm. These classification methods were employed to identify potential points for pegmatite exploration as these rocks have gained economic importance for being sources of raw materials such as high- purity quartz, ceramic feldspars, and Rare Earth Elements (REE). The high-resolution hillshade map was used to extract geological structures in the study area. The results of the fuzzy logic approach indicate potential locations of interest for pegmatite prospecting, providing a more comprehensive analysis of the remote sensing methods in the Tysfjord area. The resulting map seamlessly integrates into reports, streamlining field validation and supports informed decision-making. The methodology proposed in this study can be adaptable to other targets (minerals and rocks) and can be used as a guide for exploration worldwide.</summary>
    <dc:date>2024-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Optical fiber sensors based on sol-gel materials: design, fabrication and application in concrete structures</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/137304" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/137304</id>
    <updated>2024-12-05T07:21:33Z</updated>
    <published>2021-09-07T00:00:00Z</published>
    <summary type="text">Title: Optical fiber sensors based on sol-gel materials: design, fabrication and application in concrete structures
Abstract: Optical fiber sensing systems have been widely developed for several fields such as biomedical diagnosis, food technology, military and industrial applications and civil engineering. Nowadays, the growth and advances of optical fiber sensors (OFS) are focused on the development of novel sensing concepts and transducers as well as sensor cost reduction. This review provides an overview of the state-of-the-art of OFS based on sol-gel materials for diverse applications with particular emphasis on OFS for structural health monitoring of concrete structures. The types of precursors used in the development of sol-gel materials for OFS functionalization to monitor a wide range of analytes are debated. The main advantages of OFS compared to other sensing systems such as electrochemical sensors are also considered. An interdisciplinary review to a broad audience of engineers and materials scientists is provided and the relationship between the chemistry of sol-gel material synthesis and the development of OFS is considered. To the best of the authors' knowledge, no review manuscripts were found in which the fields of sol-gel chemistry and OFS are correlated. The authors consider that this review will serve as a reference as well as provide insights for experts into the application of sol-gel chemistry and OFS in the civil engineering field.</summary>
    <dc:date>2021-09-07T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Efficacy of selected phenolic acids in inhibiting the LasI/LasR and PQS quorum sensing pathways of Pseudomonas aeruginosa</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/162156" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/162156</id>
    <updated>2024-10-25T06:17:48Z</updated>
    <published>2024-05-01T00:00:00Z</published>
    <summary type="text">Title: Efficacy of selected phenolic acids in inhibiting the LasI/LasR and PQS quorum sensing pathways of Pseudomonas aeruginosa</summary>
    <dc:date>2024-05-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Overview of the CLEF-2024 CheckThat! Lab Task 3 on persuasion techniques</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/161555" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/161555</id>
    <updated>2024-09-19T06:18:56Z</updated>
    <published>2024-01-01T00:00:00Z</published>
    <summary type="text">Title: Overview of the CLEF-2024 CheckThat! Lab Task 3 on persuasion techniques
Abstract: We present an overview of CheckThat! Lab's 2024 Task 3, which focuses on detecting 23 persuasion techniques
at the text-span level in online media. The task covers five languages, namely, Arabic, Bulgarian, English,
Portuguese, and Slovene, and highly-debated topics in the media, e.g., the Isreali-Palestian conflict, the Russia-
Ukraine war, climate change, COVID-19, abortion, etc. A total of 23 teams registered for the task, and two of
them submitted system responses which were compared against a baseline and a task organizers' system, which
used a state-of-the-art transformer-based architecture. We provide a description of the dataset and the overall
task setup, including the evaluation methodology, and an overview of the participating systems. The datasets
accompanied with the evaluation scripts are released to the research community, which we believe will foster
research on persuasion technique detection and analysis of online media content in various fields and contexts</summary>
    <dc:date>2024-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Development of Self-Activated Photosensitizers based on the Chemiluminescent System of Marine Coelenterazine</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/159639" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/159639</id>
    <updated>2024-07-17T06:18:18Z</updated>
    <published>2024-05-28T00:00:00Z</published>
    <summary type="text">Title: Development of Self-Activated Photosensitizers based on the Chemiluminescent System of Marine Coelenterazine</summary>
    <dc:date>2024-05-28T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Utilization of multispectral satellite image to define potential tailing areas in abandoned mining areas</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/159187" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/159187</id>
    <updated>2024-06-28T06:20:18Z</updated>
    <published>2024-06-19T00:00:00Z</published>
    <summary type="text">Title: Utilization of multispectral satellite image to define potential tailing areas in abandoned mining areas</summary>
    <dc:date>2024-06-19T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>A Parametric Family of Subalgebras of the Weyl Algebra II. Irreducible Modules</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/111068" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/111068</id>
    <updated>2024-05-29T06:18:32Z</updated>
    <published>2013-01-01T00:00:00Z</published>
    <summary type="text">Title: A Parametric Family of Subalgebras of the Weyl Algebra II. Irreducible Modules
Abstract: An Ore extension over a polynomial algebra F[x] is either a quantum plane, a quantum Weyl algebra, or an infinite-dimensional unital associative algebra A(h) generated by elements x, y, which satisfy yx - xy = h, where h is an element of F[x]. We investigate the family of algebras A(h) as h ranges over all the polynomials in F[x]. When h not equal 0, the algebras A(h) are subalgebras of the Weyl algebra A(1) and can be viewed as differential operators with polynomial coefficients. We give an exact description of the automorphisms of A(h) over arbitrary fields F and describe the invariants in A(h) under the automorphisms. We determine the center, normal elements, and height one prime ideals of A(h), localizations and Ore sets for A(h), and the Lie ideal [A(h), A(h)]. We also show that A(h) cannot be realized as a generalized Weyl algebra over F[x], except when h is an element of F. In two sequels to this work, we completely describe the irreducible modules and derivations of A(h) over any field.</summary>
    <dc:date>2013-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Compositional Variations in Apatite and Petrogenetic Significance: Examples from Peraluminous Granites and Related Pegmatites and Hydrothermal Veins from the Central Iberian Zone (Spain and Portugal)</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/149881" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/149881</id>
    <updated>2023-06-02T06:23:32Z</updated>
    <published>2022-01-01T00:00:00Z</published>
    <summary type="text">Title: Compositional Variations in Apatite and Petrogenetic Significance: Examples from Peraluminous Granites and Related Pegmatites and Hydrothermal Veins from the Central Iberian Zone (Spain and Portugal)
Abstract: Apatite can be used as an archive of processes occurring during the evolution of granitic magmas and as a pegmatite exploration tool. With this aim, a detailed compositional study of apatite was performed on different Variscan granites, pegmatites and quartz veins from the Central Iberian Zone. Manganese in granitic apatite increases with increasing evolution degree. Such Mn increase would not be related to changes in the fO(2) during evolution but rather to a higher proportion of Mn in residual melts, joined to an increase in SiO2 content and peraluminosity. In the case of pegmatitic apatite, the fO(2) and the polymerization degree of the melts seem not to have influenced the Mn and Fe contents but the higher availability of these transition elements and/or the lack of minerals competing for them. The subrounded Fe-Mn phosphate nodules, where apatite often occurs in P-rich pegmatites and P-rich quartz dykes, probably crystallized from a P-rich melt exsolved from the pegmatitic melt and where Fe, Mn and Cl would partition. The low Mn and Fe contents in the apatite from the quartz veins may be attributed either to the low availability of these elements in the late hydrothermal fluids derived from the granitic and pegmatitic melts, or to a high fO(2). The Rare Earth Elements, Sr and Y are the main trace elements of the studied apatites. The REE contents of apatite decrease with the evolution of their hosting rocks. The REE patterns show in general strong tetrad effects that are probably not related to the fluids' activity in the system. On the contrary, the fluids likely drive the non-CHARAC behavior of apatite from the most evolved granitic and pegmatitic units. Low fO(2) conditions seem to be related to strong Eu anomalies observed for most of the apatites associated with different granitic units, barren and P-rich pegmatites. The positive Eu anomalies in some apatites from leucogranites and Li-rich pegmatites could reflect their early character, prior to the crystallization of feldspars. The increase in the Sr content in apatite from Li-rich pegmatites and B-P +/- F-rich leucogranites could be related to problems in accommodating this element in the albite structure, favoring its incorporation into apatite. The triangular plots sigma REE-Sr-Y and U-Th-Pb of apatites, as well as the Eu anomaly versus the TE1,3 diagram, seem to be potentially good as petrogenetic indicators, mainly for pegmatites and, to a lesser extent, for granites from the CIZ.</summary>
    <dc:date>2022-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Delivering of Proteins to the Plant Vacuole-An Update</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/82412" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/82412</id>
    <updated>2023-05-26T06:20:48Z</updated>
    <published>2014-01-01T00:00:00Z</published>
    <summary type="text">Title: Delivering of Proteins to the Plant Vacuole-An Update
Abstract: Trafficking of soluble cargo to the vacuole is far from being a closed issue as it can occur by different routes and involve different intermediates. The textbook view of proteins being sorted at the post-Golgi level to the lytic vacuole via the pre-vacuole or to the protein storage vacuole mediated by dense vesicles is now challenged as novel routes are being disclosed and vacuoles with intermediate characteristics described. The identification of Vacuolar Sorting Determinants is a key signature to understand protein trafficking to the vacuole. Despite the long established vacuolar signals, some others have been described in the last few years, with different properties that can be specific for some cells or some types of vacuoles. There are also reports of proteins having two different vacuolar signals and their significance is questionable: a way to increase the efficiency of the sorting or different sorting depending on the protein roles in a specific context? Along came the idea of differential vacuolar sorting, suggesting a possible specialization of the trafficking pathways according to the type of cell and specific needs. In this review, we show the recent advances in the field and focus on different aspects of protein trafficking to the vacuoles.</summary>
    <dc:date>2014-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Applications of Fluorescent Carbon Dots as Photocatalysts: A Review</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/147813" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/147813</id>
    <updated>2023-03-28T06:18:10Z</updated>
    <published>2023-01-12T00:00:00Z</published>
    <summary type="text">Title: Applications of Fluorescent Carbon Dots as Photocatalysts: A Review
Abstract: Carbon dots (CDs) have attracted considerable interest from the scientific community due to their exceptional properties, such as high photoluminescence, broadband absorption, low toxicity, water solubility and (photo)chemical stability. As a result, they have been applied in several fields, such as sensing, bioimaging, artificial lighting and catalysis. In particular, CDs may act as sole photocatalysts or as part of photocatalytic nanocomposites. This study aims to provide a comprehensive review on the use of CDs as sole photocatalysts in the areas of hydrogen production via water splitting, photodegradation of organic pollutants and photoreduction and metal removal from wastewaters. Furthermore, key limitations preventing a wider use of CDs as photocatalysts are pointed out. It is our hope that this review will serve as a basis on which researchers may find useful information to develop sustainable methodologies for the synthesis and use of photocatalytic CDs.</summary>
    <dc:date>2023-01-12T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Remote sensing and GIS applications in earth and environmental systems sciences</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/147627" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/147627</id>
    <updated>2023-01-27T07:16:25Z</updated>
    <published>2021-01-01T00:00:00Z</published>
    <summary type="text">Title: Remote sensing and GIS applications in earth and environmental systems sciences</summary>
    <dc:date>2021-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Gold-Mushroom Microelectrode Arrays and the Quest for Intracellular-Like Recordings: Perspectives and Outlooks</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/143836" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/143836</id>
    <updated>2022-12-06T07:25:38Z</updated>
    <published>2021-01-01T00:00:00Z</published>
    <summary type="text">Title: Gold-Mushroom Microelectrode Arrays and the Quest for Intracellular-Like Recordings: Perspectives and Outlooks
Abstract: The ongoing search to decode the brain communication system has propelled major advancements in bioelectronics research. From these, planar microelectrode arrays (MEAs) offer the possibility to record neuronal signals for extremely long periods of time and to probe fundamental principles of learning and adaptation. Unfortunately, MEAs have low neuronal coupling and are thus insensitive to sub-threshold neuronal signals such as post-synaptic responses. To surpass this major limitation, a novel category based on 3D high aspect ratio microstructures has emerged. In particular, gold-mushroom microelectrode arrays (GM mu EAs) have appeared as a solution. Their unique morphology, promoting cell engulfment without penetrating the neuron, prevents the occurrence of cell repairing mechanisms common in other microelectrodes. This new class of recording and/or stimulating devices have opened the door to the vast communication system of neuronal cells by enhancing the cell-microelectrode interface. This review compiles the fundamentals of these promising devices including their fabrication, work principles and focusing on how the different morphological parameters affect the recording performance. Although limitations exist, there is a growing interest to explore the new solutions that GM mu EAs still offer, both as an electrophysiological tool (e.g., cell guidance) or in applications such as plasmonics.</summary>
    <dc:date>2021-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Nanomaterials with high solar reflectance as an emerging path towards energy-efficient envelope systems: a review</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/137979" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/137979</id>
    <updated>2022-10-17T17:46:39Z</updated>
    <published>2021-10-12T00:00:00Z</published>
    <summary type="text">Title: Nanomaterials with high solar reflectance as an emerging path towards energy-efficient envelope systems: a review
Abstract: The application of nanomaterials in the construction field is allowing the development of smart, green, durable and more efficient buildings. Among the most widely researched nanomaterials are nanosized cool pigments, which are being enforced to achieve thermal and energy-efficient facades, with the development of high reflectance and retro-reflectance coatings. Their peculiar optical and catalytic activity turns nanomaterials into suitable candidates to be used as dark coloured high solar reflectance without affecting aesthetic characteristics, thus improving the durability of coatings. The objective of this paper is to review the state-of-the-art on the benefits of using high reflectance nanopigments as coatings in building facades and their production and synthesis processes. It is thus divided into three main topics: (i) the benefits of using nanopigments on facades, (ii) the most important nanomaterials used as cool pigments and (iii) the main methods of synthesizing nanopigments. One expects that the study of near-infrared nanopigmentation synthesis processes will be able to promote and disseminate the use of nanotechnology in construction, assessing the production problems and limitation and thus helping to disseminate new products by reducing production costs and increase availability. [GRAPHICS] .</summary>
    <dc:date>2021-10-12T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Detecting Lithium (Li) Mineralizations from Space: Current Research and Future Perspectives</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/132332" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/132332</id>
    <updated>2022-10-12T06:28:54Z</updated>
    <published>2020-01-01T00:00:00Z</published>
    <summary type="text">Title: Detecting Lithium (Li) Mineralizations from Space: Current Research and Future Perspectives
Abstract: Optical and thermal remote sensing data have been an important tool in geological exploration for certain deposit types. However, the present economic and technological advances demand the adaptation of the remote sensing data and image processing techniques to the exploration of other raw materials like lithium (Li). A bibliometric analysis, using a systematic review approach, was made to understand the recent interest in the application of remote sensing methods in Li exploration. A review of the application studies and developments in this field was also made. Throughout the paper, the addressed topics include: (i) achievements made in Li exploration using remote sensing methods; (ii) the main weaknesses of the approaches; (iii) how to overcome these difficulties; and (iv) the expected research perspectives. We expect that the number of studies concerning this topic will increase in the near future and that remote sensing will become an integrated and fundamental tool in Li exploration.</summary>
    <dc:date>2020-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Plant Reproduction: AMOR Enables Males to Respond to Female Signals</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/129012" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/129012</id>
    <updated>2022-10-12T06:29:30Z</updated>
    <published>2016-01-01T00:00:00Z</published>
    <summary type="text">Title: Plant Reproduction: AMOR Enables Males to Respond to Female Signals
Abstract: The pollen tube of flowering plants undertakes a long journey to transport two sperm cells for double fertilization. New work on pollen tube guidance has identified an arabinogalactan-derived ovular factor that primes tubes to respond to female gametophyte-secreted attraction signals.</summary>
    <dc:date>2016-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>The best CRISPR/Cas9 versus RNA interference approaches for Arabinogalactan proteins' study</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/127811" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/127811</id>
    <updated>2022-10-16T19:09:36Z</updated>
    <published>2020-01-08T00:00:00Z</published>
    <summary type="text">Title: The best CRISPR/Cas9 versus RNA interference approaches for Arabinogalactan proteins' study
Abstract: Arabinogalactan Proteins (AGPs) are hydroxyproline-rich proteins containing a high proportion of carbohydrates, widely spread in the plant kingdom. AGPs have been suggested to play important roles in plant development processes, especially in sexual plant reproduction. Nevertheless, the functions of a large number of these molecules, remains to be discovered. In this review, we discuss two revolutionary genetic techniques that are able to decode the roles of these glycoproteins in an easy and efficient way. The RNA interference is a frequently technique used in plant biology that promotes genes silencing. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (CRISPR/Cas9), emerged a few years ago as a revolutionary genome-editing technique that has allowed null mutants to be obtained in a wide variety of organisms, including plants. The two techniques have some differences between them and depending on the research objective, these may work as advantage or disadvantage. In the present work, we propose the use of the two techniques to obtain AGP mutants easily and quickly, helping to unravel the role of AGPs, surely a great asset for the future.</summary>
    <dc:date>2020-01-08T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Three Decades of Advances in Arabinogalactan-Protein Biosynthesis</title>
    <link rel="alternate" href="https://hdl.handle.net/10216/131488" />
    <author>
      <name />
    </author>
    <id>https://hdl.handle.net/10216/131488</id>
    <updated>2022-10-16T19:17:51Z</updated>
    <published>2020-01-01T00:00:00Z</published>
    <summary type="text">Title: Three Decades of Advances in Arabinogalactan-Protein Biosynthesis
Abstract: Arabinogalactan-proteins (AGPs) are a large, complex, and highly diverse class of heavily glycosylated proteins that belong to the family of cell wall hydroxyproline-rich glycoproteins. Approximately 90% of the molecules consist of arabinogalactan polysaccharides, which are composed of arabinose and galactose as major sugars and minor sugars such as glucuronic acid, fucose, and rhamnose. About half of the AGP family members contain a glycosylphosphatidylinositol (GPI) lipid anchor, which allows for an association with the outer leaflet of the plasma membrane. The mysterious AGP family has captivated the attention of plant biologists for several decades. This diverse family of glycoproteins is widely distributed in the plant kingdom, including many algae, where they play fundamental roles in growth and development processes. The journey of AGP biosynthesis begins with the assembly of amino acids into peptide chains of proteins. An N-terminal signal peptide directs AGPs toward the endoplasmic reticulum, where proline hydroxylation occurs and a GPI anchor may be added. GPI-anchored AGPs, as well as unanchored AGPs, are then transferred to the Golgi apparatus, where extensive glycosylation occurs by the action of a variety glycosyltransferase enzymes. Following glycosylation, AGPs are transported by secretory vesicles to the cell wall or to the extracellular face of the plasma membrane (in the case of GPI-anchored AGPs). GPI-anchored proteins can be released from the plasma membrane into the cell wall by phospholipases. In this review, we present an overview of the accumulated knowledge on AGP biosynthesis over the past three decades. Particular emphasis is placed on the glycosylation of AGPs as the sugar moiety is essential to their function. Recent genetics and genomics approaches have significantly contributed to a broader knowledge of AGP biosynthesis. However, many questions remain to be elucidated in the decades ahead.</summary>
    <dc:date>2020-01-01T00:00:00Z</dc:date>
  </entry>
</feed>

