DSpace Collection:https://hdl.handle.net/10216/261552026-07-18T07:16:29Z2026-07-18T07:16:29ZDectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocyteshttps://hdl.handle.net/10216/1494892023-05-24T06:28:06Z2021-01-01T00:00:00ZTitle: Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocytes
Abstract: Yeast-derived products containing β-glucans have long been used as feed supplements in domesticated animals in an attempt to increase immunity. β-glucans are mainly recognized by the cell surface receptor CLEC7A, also designated Dectin-1. Although the immune mechanisms elicited through Dectin-1 activation have been studied in detail in mice and humans, they are poorly understood in other species. Here, we evaluated the response of bovine monocytes to soluble and particulate purified β-glucans, and also to Zymosan. Our results show that particulate, but not soluble β-glucans, can upregulate the surface expression of costimulatory molecules CD80 and CD86 on bovine monocytes. In addition, stimulated cells increased production of IL-8 and of TNF, IL1B, and IL6 mRNA expression, in a dose-dependent manner, which correlated positively with CLEC7A gene expression. Production of IL-8 and TNF expression decreased significantly after CLEC7A knockdown using two different pairs of siRNAs. Overall, we demonstrated here that bovine monocytes respond to particulate β-glucans, through Dectin-1, by increasing the expression of pro-inflammatory cytokines. Our data support further studies in cattle on the induction of trained immunity using dietary β-glucans.2021-01-01T00:00:00ZTissue response to neural implants: the use of model systems towards new design solutions of implantable microelectrodeshttps://hdl.handle.net/10216/1256152020-01-24T15:59:31Z2019-08-01T00:00:00ZTitle: Tissue response to neural implants: the use of model systems towards new design solutions of implantable microelectrodes2019-08-01T00:00:00Z